Torularhodin Biosynthesis and Extraction by Yeast Cells of Rhodotorula Rubra

The paper presents the research work done to study the discontinuous bioprocess for the intracellular carotenoidic pigment torularhodin formation with the yeast Rhodotorula rubra ICCF 209. The growth and carotenoid biosynthesis of the yeast Rhodotorula rubra was studied by cultivation in 3.7 L Bioengineering AG lab bioreactor with mechanical stirring at an air flow rate of 200 L/h and agitation at 600 rpm for 4 days. The medium composition, defined as MS3, was obtained with the formula: 30 g/L glucose, 1.5 g/L yeast extract, 5 g/L NH4NO3, 1 g/L KH2PO4, 0.4 g/L MgSO4 x 7H2O, 0.4 g/L NaCl and 1 g/L alanine. The bioreactor was equipped with computerized control units for temperature, air flow, stirrer speed, pH, and pO2, and acquisition data every 15 s was programmed.